Method validation study on a HPLC-FLD based method for determination of Ochratoxin A in liquorice based food

Method validation study on a HPLC-FLD based method for determination of Ochratoxin A in liquorice based food

The European Union Reference Laboratory for Mycotoxins (EU-RL Mycotoxins) organiseD in the second half of 2010 an inter-laboratory comparison for the validation of an analytical method (MVS) to determine ochratoxin A (OTA) in liquorice root and liquorice extracts, along the requirements of the Commission Regulation No 105/2010.

The method is based on solvent extraction, immunoaffinity column purification (IAC), and detection / quantitation by high performance liquid chromatography (HPLC) coupled with fluorescence detection (FLD).

Design of the study

The MVS was conducted and evaluated according to ISO 5725-2:1994.

The participants to the study were asked to analyse a total of about 20 samples, including liquorice extract (as paste and as powder), liquorice root and candies containing liquorice extract.

The concentration levels were between 3 and 200 µg/kg.

The starting date of the comparison was decided upon the amount of laboratories expressing their interest in participating in the study. Dispatch was pre-announced at least two weeks in advance.

The participants had 6 weeks from dispatch date to report back the results.

The MVS was open to all interested laboratories, e.g. NRLs, official food control laboratories, private food control laboratories, academia, and laboratories from industry.

For more information about this MVS, please contact:

Donata Lerda

Institute for Reference Materials and Measurements (IRMM)

EU-RL Mycotoxins

Retieseweg 111

B-2440 Geel, Belgium

Tel: +32-14-571 826

Fax: +32-14-571 783

E-mail: jrc-irmm-crl-mycotoxec [dot] europa [dot] eu (subject: ) (jrc-irmm-crl-mycotox[at]ec[dot]europa[dot]eu)

Year:	2010-01-01 00:00:00
Contact:	jrc-irmm-crl-mycotoxec [dot] europa [dot] eu (jrc-irmm-crl-mycotox[at]ec[dot]europa[dot]eu)