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Lactococcus lactis DSM 34262

Lactococcus lactis DSM 34262


Publication date
29 August 2023 (Last updated on: 28 February 2024)
Joint Research Centre


In the current application an authorisation is sought under Article 4(1) for Lactococcus lactis DSM 34262 under the category / functional group 1(k) 'technological additives' / 'silage additives', according to Annex I of Regulation (EC) No 1831/2003. The authorisation is sought for the use of the feed additive for all animal species.
According to the Applicant, the feed additive contains the non-genetically modified strain Lactococcus lactis DSM 34262 as active substance at a minimum level of 3 x 1011 Colony Forming Unit (CFU) / g. The feed additive is intended to be added to the forage, used for ensiling process at a minimum recommended dosage of 1 x 105 CFU /g fresh silage.
For the enumeration of Lactococcus lactis DSM 34262 in the feed additive and silage the Applicant applied an in-house pour-plate method based on the ISO 15214 pour-plate method.
Based on the available information, the EURL recommends for official control the ISO 15214 method for the enumeration of Lactococcus lactis DSM 34262 in the feed additive per se.
Furthermore, for the identification of Lactococcus lactis DSM 34262 at a strain level, the EURL recommends for official control (i) DNA sequencing methods (e.g. Whole Genome Sequencing (WGS)) or (ii) Pulsed-Field Gel Electrophoresis (PFGE).
As the enumeration of added Lactococcus lactis DSM 34262 in silage is not achievable by analysis, the EURL cannot recommend any method for official control.
Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.


29 AUGUST 2023