Details
- Publication date
- 29 November 2021 (Last updated on: 9 December 2021)
Description
In the current application an authorisation is sought under Article 4(1) (new feed additive) for a preparation of Propionibacterium freudenreichii DSM 33189 and Lactobacillus buchneri DSM 12856 under the category/functional group 1(k) "technological additives"/"silage additives", according to Annex I of Regulation (EC) No 1831/2003. The authorisation is sought for the use of the feed additive for all animal species.
According to the Applicant, the feed additive contains a non-genetically modified strains of Propionibacterium freudenreichii DSM 33189 and Lactobacillus buchneri DSM 12856 as active substances with a minimum content of 1 x 1011 and 4 x 1011 Colony Forming Units (CFU) / g feed additive, respectively.
The feed additive is intended to be added into silage through its aqueous suspension at a minimum dose of the active substances of 1 x 108 CFU / kg fresh silage.
For the genetic identification of Propionibacterium freudenreichii DSM 33189 and Lactobacillus buchneri DSM 12856 the EURL recommends for official control the pulsed-field gel electrophoresis (PFGE), a generally recognised methodology for the genetic identification of bacterial strains.
For the enumeration of Lactobacillus buchneri DSM 12856 in the feed additive the EURL recommends for official control the ring-trial validated EN 15787 method.
For the enumeration of Propionibacterium freudenreichii DSM 33189 in the feed additive the EURL recommends for official control the pour plate method on caseine peptone, yeast extract, sodium lactate and L-cysteine agar (based on ISO 27205 standard method).
Since the unambiguous enumeration of content of Propionibacterium freudenreichii DSM 33189 and Lactobacillus buchneri DSM 12856 initially added to silage is not experimentally achievable, the EURL is not able to evaluate or recommend any method for official control for the enumeration of these microorganisms in silage.
Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.
Recommended Method
as of:
- Commission Implementing Regulation (EU) 2022/1382
For the identification of Propionibacterium freudenreichii DSM 33189 and Lentilactobacillus buchneri DSM 12856:
— Pulsed-Field Gel Electrophoresis (PFGE) or DNA sequencing methods
For the enumeration of Lentilactobacillus buchneri DSM 12856 in the feed additive:
— Spread plate method on MRS agar (EN 15787)
For the enumeration of Propionibacterium freudenreichii DSM 33189 in the feed additive:
— Pour plate method on caseine peptone, yeast extract, sodium lactate and L-cysteine agar