Pediococcus pentosaceus IMI 507024 - 1k21016, Pediococcus pentosaceus IMI 507025 - 1k21017

In the current applications authorisations are sought under Article 4(1) for Pediococcus pentosaceus IMI 507024 (FAD 2020-0076) and for Pediococcus pentosaceus IMI 507025 (FAD 2020-0077) under the category/functional group 1(k) "technological additives"/"silage additives", according to Annex I of Regulation (EC) No 1831/2003. Specifically, the authorisations are sought for the use of the feed additives in silage for all animal species. 

According to the Applicant, the feed additives contain as active substance viable cells of the strains Pediococcus pentosaceus IMI 507024 or Pediococcus pentosaceus IMI 507025. The feed additives are to be marketed as preparations with a minimum content of 1x10¹⁰ Colony Forming Units (CFU) of Pediococcus pentosaceus IMI 507024 or Pediococcus pentosaceus IMI 507025 / g feed additive. Both feed additives are intended to be used at a minimum dose of 1x10⁶ CFU/kg fresh silage. 

For the identification of Pediococcus pentosaceus IMI 507024 and Pediococcus pentosaceus IMI 507025, the EURL recommends for official control Pulsed-Field Gel Electrophoresis (PFGE), a generally recognised methodology for the genetic identification of bacterial strains.

For the enumeration of Pediococcus pentosaceus IMI 507024 and Pediococcus pentosaceus IMI 507025 in the feed additives, the EURL recommends for official control the ring-trial validated spread plate method EN 15786. 

Since the unambiguous determination of the content of Pediococcus pentosaceus IMI 507024 or Pediococcus pentosaceus IMI 507025 initially added to silage is not achievable by analysis, the EURL cannot evaluate nor recommend any method for official control for the determination of Pediococcus pentosaceus IMI 507024 or Pediococcus pentosaceus IMI 507025 in silage. 

Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.

Recommended Method

as of:

Commission Implementing Regulation (EU) 2022/273 

Identification: Pulsed-Field Gel Electrophoresis (PFGE)

Enumeration in the feed additives: Spread plate method on MRS agar (EN 15786)