L. rhamnosus IMI 507023 - 1k21701, L. plantarum IMI 507026 - 1k21601, L. plantarum IMI 507027 - 1k21602, L. plantarum IMI 507028 - 1k21603

In the current applications authorisations are sought under Article 4(1) for Lactobacillus rhamnosus IMI 507023, Lactobacillus plantarum IMI 507026, Lactobacillus plantarum IMI 507027 and Lactobacillus plantarum IMI 507028 under the category/functional group 1(k) "technological additives"/"silage additives", according to Annex I of Regulation (EC) No 1831/2003. The authorisations are sought for the use of the feed additives for all animal species. 

According to the Applicant, the feed additives contain as active substance viable cells of Lactobacillus rhamnosus IMI 507023, Lactobacillus plantarum IMI 507026, Lactobacillus plantarum IMI 507027 and Lactobacillus plantarum IMI 507028, respectively. The feed additives are to be marketed as preparations containing a minimum content of 1x10¹⁰ Colony Forming Units (CFU) of Lactobacillus rhamnosus IMI 507023 or Lactobacillus plantarum IMI 507026 or Lactobacillus plantarum IMI 507027 or Lactobacillus plantarum IMI 507028/g in the respective feed additive. The feed additives are intended to be used at a minimum dose of 1x10⁶ CFU/kg fresh silage. 

For the identification of Lactobacillus rhamnosus IMI 507023, Lactobacillus plantarum IMI 507026, Lactobacillus plantarum IMI 507027 and Lactobacillus plantarum IMI 507028 the EURL recommends for official control Pulsed-Field Gel Electrophoresis (PFGE), a recognised methodology for the genetic identification of bacterial strains. 

For the enumeration of Lactobacillus rhamnosus IMI 507023, Lactobacillus plantarum IMI 507026, Lactobacillus plantarum IMI 507027 and Lactobacillus plantarum IMI 507028 in the feed additives the EURL recommends for official control the ring-trial validated spread plate method EN 15787. 

Since the unambiguous determination of the content of Lactobacillus rhamnosus IMI 507023 or Lactobacillus plantarum IMI 507026 or Lactobacillus plantarum IMI 507027 or Lactobacillus plantarum IMI 507028 initially added to silage is not experimentally achievable, the EURL is not able to evaluate or recommend any method for official control for the determination Lactobacillus rhamnosus IMI 507023, Lactobacillus plantarum IMI 507026, Lactobacillus plantarum IMI 507027 and Lactobacillus plantarum IMI 507028 in silage. 

Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.

Recommended Method

as of:

Commission Implementing Regulation (EU) 2022/273 

Identification: Pulsed-Field Gel Electrophoresis (PFGE)

Enumeration in the feed additives: Spread plate method on MRS agar (EN 15787)