Loigolactobacillus coryniformis DSM 34345

In the current application an authorisation is sought under Article 4 for Loigolactobacillus coryniformis DSM 34345 under the category / functional group 1(k) 'technological additives' / 'silage additives', according to Annex I of Regulation (EC) No 1831/2003. The authorisation is sought for the use of the feed additive for all animal species.
According to the Applicant, the feed additive contains the non-genetically modified Loigolactobacillus coryniformis DSM 34345 as active substance at a minimum concentration of 2 x 10¹¹ Colony Forming Unit (CFU) / g. The feed additive is intended to be added to the forage used for ensiling process at a recommended dosage of 1 x 10⁵ CFU / g fresh silage.
For the enumeration of Loigolactobacillus coryniformis DSM 34345 in the feed additive and in silage the Applicant proposed the ring-trial validated EN 15787 method. As the unambiguous determination of Loigolactobacillus coryniformis DSM 34345 added to silage is not achievable by analysis, the EURL cannot evaluate nor recommend any method for official control. Based on the performance characteristics, as already concluded for similar Lactoplantibacilli reports, the EURL recommends for official control the ring-trial validated EN 15787 method for the enumeration of Loigolactobacillus coryniformis DSM 34345 in the feed additive.
For the identification of Loigolactobacillus coryniformis DSM 34345, the Applicant applied a DNA sequencing method such as Whole Genome Sequencing (WGS). In former reports for similar dossiers, the EURL recommended for official control DNA sequencing methods or Pulsed-Field Gel Electrophoresis (PFGE) described in CEN Technical Specification (CEN/TS 17697). The EURL considers that all the above-mentioned methodologies are suitable for official control for the bacterial identification of Loigolactobacillus coryniformis DSM 34345 at strain level.
Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.