Details
- Publication date
- 28 January 2013
Description
Analysis of aflatoxins B1, B2, G1 and G2 in acetonitrile
The aim of this study is to provide national reference laboratories (NRLs) with the possibility to assess their instrument calibration for the analysis of aflatoxins B1, B2, G1 and G2 in acetonitrile.
Participation is restricted to national reference laboratories for mycotoxins.
Test materials and analytes
Participants will receive a set of standard solutions of aflatoxin B1, B2, G1 and G2 in actonitrile; each standard solution contains only one of the aflatoxins. Two ampoules per standard will be supplied, in total 8 ampoules (4 x 2) as specified on the label. The individual aflatoxins are at a concentration level ranging between 1 to 10 μg/mL. Each ampoule contains approximately 4 mL of solution.
The target analytes are:
Aflatoxin B1 in acetonitrile | Aflatoxin B2 in acetonitrile |
Aflatoxin G1 in acetonitrile | Aflatoxin G2 in acetonitrile |
General outline of the exercise
The participants are requested to perform triplicate analyses on each of the ampoules applying an HPLC method of their own choice. The analysis of the two ampoules per individual aflatoxin shall be performed on two different days as illustrated in the following tables:
day 1 = ampoule xx | |||
---|---|---|---|
aflatoxin | replicate 1 | replicate 2 | replicate 3 |
B1 | |||
B2 | |||
G1 | |||
G2 |
day 2 = ampoule yy | |||
---|---|---|---|
aflatoxin | replicate 1 | replicate 2 | replicate 3 |
B1 | |||
B2 | |||
G1 | |||
G2 |
The national reference laboratories are kindly requested to fill in a method questionnaire and report back the results of the analyses using the reporting grid at
http://www.irmm.jrc.be/imepapp/registerForComparison.action?comparison=80
This web based interface will be open by 24 November 2006.
Please send also a signed hard-copy of the reporting grid, together with representative chromatograms by regular mail to:
Ivanka Doncheva-Tsaneva
European Commission
Directorate-General Joint Research Centre
Institute for Reference Materials and Measurements
Retieseweg 111
B-2440 Geel, Belgium
Year: | 2007-01-01 00:00:00 |