Lactobacillus diolivorans DSM 33625

In the current application an authorisation is sought under Article 4 for Lactobacillus diolivorans DSM 33625 under the category / functional group 1(k) 'technological additives' / 'silage additives', according to Annex I of Regulation (EC) No 1831/2003. The authorisation is sought for the use of the feed additive for all animal species.
According to the Applicant, the feed additive contains as active substance viable cells of the non-genetically modified strain Lactobacillus diolivorans DSM 33625 at a minimum level of 2x1011 Colony Forming Unit (CFU)/g. The feed additive is intended to be added to silage at a minimum dose of 1x105 CFU/g fresh silage.
For the enumeration of Lactobacillus diolivorans DSM 33625 in the feed additive per se the Applicant proposed for official control the ring-trial validated spread plate (or pour plate) method EN 15787.
Based on the performance characteristics, as already concluded for similar Lactobacilli reports, the EURL recommends for official control the ring-trial validated EN 15787 method for the enumeration of Lactobacillus diolivorans DSM 33625 in the feed additive per se.
Furthermore, for the identification of Lactobacillus diolivorans DSM 33625 at a strain level, the EURL recommends for official control (i) DNA sequencing methods (e.g. Whole Genome Sequencing (WGS)) or (ii) Pulsed-Field Gel Electrophoresis (PFGE).
As the enumeration of added Lactobacillus diolivorans DSM 33625 in silage is not achievable by analysis, the EURL cannot recommend any method for official control.
Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.