Lactiplantibacillus plantarum DSM 26571 - 1k1604

In the current application an authorisation is sought under Article 4(1) for Lactobacillus plantarum DSM26571 under the category / functional group 1(k) 'technological additives' / 'silage additives', according to Annex I of Regulation (EC) No 1831/2003. The authorisation is sought for the use of the feed additive for all animal species. 

According to the Applicant, the feed additive contains as active substance viable cells of the non-genetically modified strain Lactobacillus plantarum DSM26571. The feed additive is to be marketed as a preparation containing a minimum content of active substance of 1x10¹¹ Colony Forming Unit (CFU)/g and to be added to silage as such or mixed with other additives, at a minimum dose of 1x10⁵ CFU/g fresh silage. 

For the identification of Lactobacillus plantarum DSM26571 the EURL recommends for official control Pulsed-Field Gel Electrophoresis (PFGE), a generally recognised methodology for the genetic identification of bacterial strains. 

For the enumeration of Lactobacillus plantarum DSM26571 in the feed additive the EURL recommends for official control the ring-trial validated spread plate method EN 15787. 

Since the enumeration of Lactobacillus plantarum DSM26571 initially added to silage is not achievable by analysis, the EURL cannot recommend the method EN 15787 or any other method for official control to enumerate Lactobacillus plantarum DSM26571 in silage.

Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005), as last amended by Regulation (EU) 2015/1761) is not considered necessary.

Recommended Method

as of:

Commission Implementing Regulation (EU) 2022/633 

Identification: Pulsed-Field Gel Electrophoresis (PFGE)

Enumeration in the feed additive: Spread plate method on MRS agar (EN 15787)