Companilactobacillus farciminis CNCM I-3740

In the current application authorisation is sought under Article 10(2) for Lactobacillus farciminis (CNCM MA67/4R)¹ under the category / functional group 4(b) 'zootechnical additives' / 'gut flora stabilisers', according to Annex I of Regulation (EC) No 1831/2003. Authorisation is sought for the use of the feed additive for chickens and turkeys for fattening and laying hens. This feed additive is currently authorised by Regulation (EC) No 1876/2006. 

According to the Applicant, the feed additive contains as active substance Lactobacillus farciminis (CNCM MA67/4R). The feed additive is to be marketed with a minimum Lactobacillus farciminis (CNCM MA67/4R) content of 109 Colony Forming Unit (CFU)/g. It is intended to be incorporated through premixtures at a minimum content of 5 x 10⁸ CFU/kg feedingstuffs. 

For the identification of Lactobacillus farciminis (CNCM MA67/4R) the Applicant applied partial 16S rDNA gene sequence analysis. The EURL recommends instead for official control Pulsed Field Gel Electrophoresis (PFGE), a generally recognised methodology for genetic identification of bacterial strains. 

For the enumeration of Lactobacillus farciminis (CNCM MA67/4R) in the feed additive and feedingstuffs, the Applicant applied an amended French Standard (NF V04-503, 1988). The EURL recommends instead for official control the ring-trial validated spread plate method EN 15787. 

Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.

¹New name: Companilactobacillus farciminis CNCM I-3740

Recommended Method

as of:

- Commission Implementing Regulation (EU) 2024/228

Identification:

• Pulsed Field Gel Electrophoresis (PFGE) – CEN/TS 17697 

  or

• DNA sequencing methods

Enumeration in feed additive and compound feed: 

• Spread plate method on MRS agar (EN 15787)