- Publication date
- 25 November 2022 (Last updated on: 28 February 2023)
- Joint Research Centre
In the current application an authorisation is sought under Article 4(1) for Streptococcus salivarius K12 under the category / functional group 1(j) 'technological additives' / 'acidity regulators', according to Annex I of Regulation (EC) No 1831/2003. The authorisation is sought for the use of the feed additive for cats, dogs and other non-food producing animals.
The active substance of the feed additive is Streptococcus salivarius K12 strain with a minimum content of 1.0x1011 Colony Forming Unit (CFU) / g product.
The feed additive is intended to be used directly in liquid feedingstuffs with a minimum proposed dose of the active substance of 1.0x108 CFU / ml feedingstuffs. The feed additive is not intended for use in dry vitamin/mineral premixtures.
For the enumeration of Streptococcus salivarius K12 in the feed additive and feedingstuffs the Applicant submitted single-laboratory validated and further verified spread plate (or spiral plate) methods on Columbia Blood Agar containing yeast extract, glucose and calcium carbonate.
Based on the performance characteristics and all available information, the EURL recommends for official control the single-laboratory validated and further verified spread plate (or spiral plate) methods on Columbia Blood Agar containing yeast extract, glucose and calcium carbonate for the enumeration of Streptococcus salivarius K12 in the feed additive and feedingstuffs.
Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.