- Publication date
- 1 April 2022 (Last updated on: 8 July 2022)
- Joint Research Centre
In the current application an authorisation is sought under Article 4 for endo-1,4-beta-mannanase under the category / functional group 4(a) "zootechnical additives / digestibility enhancers", according to the classification system of Annex I of Regulation (EC) No 1831/2003. Specifically, the authorisation is sought for chickens for fattening and other poultry for fattening.
According to the Applicant, the feed additive contains as active substance endo-1,4-beta-mannanase produced by the non-genetically modified microorganism Aspergillus niger (CBS 120604).
The feed additive is intended to be marketed under the trade name Nutrixtend Optim as a solid preparation with a minimum endo-1,4-beta-mannanase activity of 265 units (U) / g feed additive.
According to the Applicant, one mannanase unit (U) is defined as “the amount of enzyme required to release one micromole of mannose reducing sugar equivalents per minute at 40 oC and pH 4.5”.
The feed additive is intended to be used directly into feedingstuffs or through premixtures at a recommended mimimum endo-1,4-beta-mannanase activity of 27 U / kg complete feedingstuffs.
For the quantification of the endo-1,4-beta-mannanase activity in the feed additive, premixtures and feedingstuffs, the Applicant submitted a single-laboratory validated and further verified colorimetric method based on the quantification of the water soluble dyed fragments produced by the action of endo-1,4-beta-mannanase on a commercially available (Beta-Mannazyme, Megazyme) azurine-crosslinked-carob galactomannan substrate.
Based on the overall available performance data, the EURL recommends for official control the colorimetric (Megazyme) method for the determination of the endo-1,4-beta-mannanase activity in the feed additive, premixtures and feedingstuffs.
Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by Article 10 (Commission Regulation (EC) No 378/2005, as last amended by Regulation (EU) 2015/1761) is not considered necessary.