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Guanidinoacetic acid (3c372)

Guanidinoacetic acid

Details

Publication date
29 April 2013 (Last updated on: 6 June 2016)

Description

Recommended Method

as of:

- Commission Implementing Regulation (EU) 2016/1768

For the determination of guanidinoacetic acid in the feed:

- ion chromatography coupled with ultraviolet detection (IC-UV)

m1-fa_fs-3c372.pdf
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(566.64 KB - PDF)
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Executive Summary

Guanidinoacetic acid (GAA) is already authorised as feed additive for chickens for fattening. In the current application authorisation is sought under Article 4(1) for guanidinoacetic acid under the category/functional group 3(c) 'nutritional additives'/'amino acids, their salts and analogues', according to Annex I of Regulation (EC) No 1831/2003. Authorisation is sought for all animal species. For the determination of GAA in the feed additive and feedingstuffs the Applicant proposed a single laboratory validated and further verified method based on Ion Chromatography coupled to UltraViolet detection (IC-UV). The performance characteristics recalculated by EURL, based on the experimental data provided by the Applicant, are : a relative standard deviation for repeatability (RSDr) ranging from 0.11 to 4.2 %; a recovery rate (RRec) ranging from 96.4 to 99.1 %; and a limit of quantification (LOQ) in the feedingstuffs of 50 mg/kg. For the determination of GAA in premixtures the Applicant proposed a single laboratory validated method based on High Performance Liquid Chromatography coupled to UltraViolet (HPLC-UV), which is very similar to the method applied for the feed additive and feedingstuffs. The Applicant reported RSDr values ranging from 0.6 to 1.4% and RRec values ranging from 96 to 108%. Based on the performance characteristics presented, the EURL recommends for official control (1) the single laboratory validated and further verified method based on ion chromatography coupled to ultraviolet detection to determine guanidinoacetic acid in the feed additive and feedingstuffs and (2) the single laboratory validated to determine guanidinoacetic acid in the premixture. Further testing or validation of the methods to be performed through the consortium of National Reference Laboratories as specified by article 10 (Commission Regulation (EC) No 378/2005) is not considered necessary.

Files

FinRep-FAD-2011-0043-GAA.doc_.pdf
English
(80.11 KB - PDF)
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