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Artikkel24 september 2018

Understanding protein interaction when human cells are exposed to silver nanoparticles

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Caco-2 cells exposed to 10 μg/mLAgNPs for 72 h - JRC Nanobiotechnology Laboratory
© EU, 2018

JRC scientists applied proteomics for the assessment of the potential human health risks of silver nanoparticles. The knowledge gained will contribute to the understanding of interaction of proteins with nanoparticles and supports the development of safe nanomaterials for use in consumer products or medical applications.

Silver nanoparticles are the most commercialized nanomaterials on the market with over 400 registered applications to date. It is however important to understand the fate and potential impacts on the biological systems.

Due to their size, nanoparticles can readily penetrate the body and cells through various routes. The Caco-2 human epithelial cell line is one of the most relevant in vitro models to study intestinal functions and therefore serves often for toxicity assessment.

JRC scientists used two complementary proteomic approaches to understand the interactions of silver nanoparticles with Caco-2 cells.

They were able to detect significant changes in the protein profiles of Caco-2 cells treated with 30 nm silver nanoparticles over a day to three days exposure.

The mechanistic understanding of silver nanoparticle toxicity will contribute to a more effective assessment of the growing number of new nanomaterials and may ultimately contribute to safe products on the market. Moreover, this study contributes to the accumulation of data in the public domain, with the potential to generate new knowledge.

Read more in: S. Gioria et al.: Proteomics study of silver nanoparticles on Caco-2 cells, Toxicol. in Vitro 50 (2018) 347-372 https://doi.org/10.1016/j.tiv.2018.03.

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24 september 2018